There's no harm in switching fuels back and forth as long as it's FF compatible; your 2003 truck has a physical alcohol sensor that tells the ECM to adjust the fuel delivery. Your truck will use more fuel on E85 but should be a bit peppier. Higher percentages of ethanol can cause harder cold weather starts, if you're in that type of climate.
When you change your fuel composition significantly by refilling your tank, the ECU will sense the change if 3 gallons or more are added. What I've read is then it helps to drive it 7 miles without stopping the engine; that way the fuel trims are fully adjusted. That may not be your case since you have the physical sensor (the later models have a "virtual" sensor and may need that time to adjust), and I've noticed that mine adjusts within two or three miles.
And, of course, the amount of ethanol that you're getting from the pump can vary by station and season.