What is responsible for better fuel mileage at the same * light throttle opening during cruise* ??........TIMING
The more timing without knock/detonation the more torque the better the mileage.
What allows more timing without KR? (Knock retard)
Cooler intake air.... IAT , allows more timing.....look at a tune map timing vs IAT
Higher octane fuel....allows more timing to be utilized
Lower engine temp ECT ....less prone to knock/det
Your highest amount of timing will be at the lightest load/throttle like cruising at 40 mph.
OEM tuning is for the minimum fuel octane available everywhere, or for performance vehicles they stipulate in the manual the minimum requirement.
Aftermarket tuning can give you more timing if you are dedicated to using high octane fuel, add a real cold air intake and its even better.
PS, you cant really do MPG comparison with gas vs E85 as it has ~27% less energy than gas....the only good thing is the higher octane rating. You would have to do a price comparison with total miles driven.