For me, without actually testing the ethanol content of the fuel I purchased, I am just happy to see anything under 10% ethanol content since that is what I am purchasing.
It would definitely be more accurate if I bought one of those ethanol test kits and actually checked the fuel but I don't really care that much as long as it continues to calculate at something under 10%.
I will continue to post my calculated ethanol percentage over the next several fill ups so we can see if this was the actual fix.
Having used my test bottle on a number of samples so far, I have come to a few conclusions.
First, the gas stations are pretty reliable as far as not having more than 10% Ethanol in E10, in fact, what I have tested is they are generally pretty close to 5-6%, definitely not something to think about being more than 10% as a general rule.
Second, the reason to buy a test bottle, other than for testing when something is wrong with your flex fuel vehicle and you have ruled out a lot of more obvious candidates, is to check how much gasoline is in your e85.
The variability of ethanol content in e85 seems far greater than the variability of ethanol in e10 (by geometric proportions), so a test bottle might actually tell you something meaningful testing e85.
One thing I plan next is to try and quit focusing on how far off my virtual sensor is measuring e10 and start seeing how off it is measuring e85. I will be filling up with e85 for a few tanks and then also doing the bottle test. I am guessing, given my primitive fuel pressure management setup in my 2007, that the algorithms are optimized to guess more accurately at ethanol content of e85 versus e10. My theory will be confirmed if it gets a lot closer in percentage terms versus measuring e10.